Peer-reviewed publications across structural biology
GPCR · Virus · Gene Editing · Antibody · Photosynthesis · Cilia/Flagella
Shanghai Institute of Materia Medica, CAS · Xu H et al.
ANTcryo™ and UltrAuFoil grids were used to reveal the Omicron mutant strain spike protein structure binding to the therapeutic antibody JMB2002 and receptor ACE2. The final resolutions achieved were 2.69 Å (using ANTcryo™) and 2.77 Å (using UltrAuFoil), demonstrating superior resolution performance.
Stanford University
Comparative analysis using ANTcryo™ and Quantifoil grids for cryo-EM sample preparation of adrenergic receptors. The study achieved resolutions of 2.49 Å with ANTcryo™ and 2.99 Å with Quantifoil, demonstrating superior performance of ANTcryo™ technology.
ShanghaiTech University · Liu ZJ et al.
Three-dimensional cryo-EM structures of CB1 and CB2 cannabinoid receptors bound to G protein complexes. Sample preparation used ANTcryo™ grids (formerly CryoMatrix). Final resolutions: CB1 at 3.0 Å, CB2 at 2.9 Å.
Wang T, Liu ZJ et al. · ShanghaiTech University, iHuman Institute
The human olfactory receptor OR6A2 was successfully engineered into a functional protein for the first time, revealing its reversible covalent bond recognition mechanism for aldehyde odorants. A broadly conserved activation switch was discovered across Class II olfactory receptors.
S0092-8674(25)01430-8Xing L, Lu L et al. · Fudan University
First high-resolution structure of the early fusion intermediate of coronavirus S protein, revealing the precise conformation of the fusion peptide inserted into the host membrane after S2′ cleavage. Identifies this intermediate as an optimal antiviral target window.
DOI: 10.1016/j.cell.2025.01.012Xu P, Saito M, Zhang F et al. · Broad Institute / MIT
Structures of 13 Fanzor proteins from 3 different organisms reveal the molecular diversity of eukaryotic gene-editing systems. The ωRNA binding interface is highly conserved, while TAM recognition and catalytic sites vary. The RuvC domain "Lid" loop undergoes conformational change upon guide-DNA pairing to regulate activation.
S0092-8674(24)00844-4Rosen LE, Starr TN et al. (53 authors) · University of Utah / Vir Biotechnology
Discovery of human monoclonal antibody VIR-7229 with unprecedented cross-reactivity against all sarbecovirus clades, including non-ACE2 bat sarbecoviruses, while potently neutralizing all SARS-CoV-2 variants since 2019. VIR-7229 tolerates extreme epitope diversification via high-affinity binding, receptor molecular mimicry, and backbone interactions.
DOI: 10.1016/j.cell.2024.09.026Yan N, Yan C, Pan J et al. · Tsinghua University
First native structure of Chlamydomonas flagellar mastigonemes, revealing a complex assembly mediated by polysaccharides. Elucidates the molecular basis of ciliary motility and mechanosensation — the only cilia/flagella structural biology work in the collection.
DOI: 10.1016/j.cell.2024.03.005Liu ZJ et al. · ShanghaiTech University
Three-dimensional cryo-EM structures of CB1 and CB2 bound to G protein molecules. Cryo-EM sample preparation used the ANTcryo™ grid. Final resolutions: 3.0 Å and 2.9 Å.
DOI: 10.1016/j.cell.2020.01.007Jin W, Xu RM et al. · Institute of Biophysics, CAS
Reveals the DNA targeting mechanism of human LINE-1, the only autonomously active retrotransposon. ORF2p functions as a structure-dependent endonuclease, binding upstream dsDNA and recognizing downstream fork/flap structures, suggesting L1 transposition "piggybacks" on chromosomal processes with non-canonical DNA structures.
DOI: 10.1126/science.adu3433Shen L, Wang W et al. · Institute of Botany, CAS
Coccolithophore PSI-fucoxanthin supercomplex at 2.79 Å resolution — 38 peripheral FCPI antennas, 819 pigment molecules, 95% quantum efficiency. Demonstrates ANTcryo™ capability for resolving ultra-large membrane protein complexes.
DOI: 10.1126/science.adv2132Peng R, Chang YW et al. · University of Pennsylvania
First revelation of influenza virus RNP as a right-handed antiparallel double helix with viral RNA wrapped in the minor groove. Visualized conformational changes of viral polymerase across functional states; nucleoprotein tail loop identified as a key drug target with candidate lead compounds.
DOI: 10.1126/science.adq7597Xu H et al. · Shanghai Institute of Materia Medica, CAS
ANTcryo™ and UltrAuFoil grids were used to reveal the Omicron mutant strain spike protein structure binding to therapeutic antibody JMB2002 and receptor ACE2. Resolutions: 2.69 Å (ANTcryo™) vs 2.77 Å (UltrAuFoil).
Wu B et al. · Shanghai Institute of Materia Medica, CAS
Cryo-EM structures of the glucagon receptor in complex with Gs and various ligands, providing a structural framework for understanding class B GPCR activation and rational drug design.
DOI: 10.1126/science.aaz5346Saito M, Xu P, Faure G et al. · Broad Institute of MIT and Harvard
First biochemical and structural characterization of Fanzor (Fz) proteins as eukaryotic RNA-guided DNA endonucleases — the evolutionary link between prokaryotic TnpB/Cas12 and eukaryotic gene regulation. The 2.7 Å cryo-EM structure reveals a conserved catalytic core despite diverse guide RNAs. Fanzor can be reprogrammed for human genome engineering, demonstrating that RNA-guided endonucleases exist across all three domains of life.
DOI: 10.1038/s41586-023-06356-2Duan J, Liu H, Zhao F et al. · Shanghai Institute of Materia Medica, CAS
First complex structure of neurotensin receptor 1 (NTSR1) bound to GRK2, Gαq, and the arrestin-biased ligand SBI-553. GRK2 N-terminal helix docks into the open receptor cytoplasmic pocket formed by TM6 outward movement, analogous to G-protein binding. SBI-553 binds at the GRK2–NTSR1 interface, compatible with arrestin but clashing with Gαq — providing the structural basis for GRK2-mediated biased signaling.
DOI: 10.1038/s41586-023-06395-9Kobilka B et al. · Stanford University
A novel calcineurin-fusion strategy for GPCR structural biology, enabling high-resolution cryo-EM studies. Represents the first ANTcryo™ publication from a leading international structural biology lab.
DOI: 10.1073/pnas.2414544121Sun D, Xu HE, Tian C et al. · University of Science and Technology of China
First high-resolution structure (2.65–2.88 Å) of a GPCR–β-arrestin1–biased allosteric modulator SBI-553 ternary complex. Discovered a novel "loop engagement" coupling mode — NTSR1 ICL3 binds the central crest cavity of β-arrestin1, representing a previously unobserved arrestin-selective GPCR conformation.
DOI: 10.1038/s41422-025-01095-7Huang F et al. · Institute of Biophysics, CAS
The foundational paper introducing the amorphous nickel-titanium alloy (ANTA) film as a revolutionary non-carbon support for cryo-EM. Demonstrated 18× lower protein adsorption, superior conductivity, and 2.36 Å resolution.
Data sourced from public ANTcryo™ user achievement records and verified against publisher databases. ANTcryo™ has supported dozens of high-impact publications — this list represents a curated selection.